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Microscope Accessories For Direct-View 3D Imaging
EdgeŽ 3D Imaging Accessories Afford Low Cost, 3D Imaging and Analysis Technology That Solve Significant Application Problems. Features Include:
Applications The applications for 3D microscope technology are extensive. The most common applications include bio-technology, autoradiology, biochemistry, botany, brain mapping, cell biology, computer analysis, dentistry, orthopedics, developmental biology, forensics, fluid inclusions, food technology,fracture analysis, geology and micro fossils, immunolabeling, inspection, in situ hybridization, in vitro, marine biology, material sciences, morphology, micromanipulation, neuroscience, Pap smears, pathology, paleontology, parisitology, physiology, plant biology, quality control, research, semiconductor, thick sections, tissue engineering, whole mounts, and any applications requiring the best possible technology in three dimensional light microscopy. Use EdgeŽ products to intuitively understand structures within layers and recognize structures above and below the x/y plane. Distinguish holes from valleys and inclusions from surface contamination. Retrofit an inverted microscope for micro-manipulation, micro-recording and patch clamping to easily locate a pipette or electrode. Use with inverted or upright microscopes to instantly understand cell morphology and differentiation in 3-dimentional tissue culture. EdgeŽ products are ideal tools for examining thick sections in neuroscience, or where the integrity of the specimen would be lost by thin sectioning. In developmental biology, thick whole mount preparations benefit from the improvement in image quality, along with the more comprehensive understanding of 3-dimentional structures. Reconstruction microscopy methods will not work with alive and moving specimens. Use EdgeŽ technology instead! 3D is Better Than 2D Conventional high power 2D microscopes occlude or obscure critical information about 3D structures, often leading professionals , researchers and technicians to misinterpret specimens. Reliance on 2D images results in lost critical information and distorts reality. EdgeŽ 3D Imaging Compared to Confocal In addition to high cost, confocal microscopes have significant limitations. Confocal microscopes typically do not provide easy access to 3D images, and usually render in-focus large depth of field in 2D images. 3D confocal is not a real-time process. After multiple image capture and processing, it requires up to several hours to acquire a volumetric image. This is not acceptable for moving objects, such as live specimens, and significantly limits the utility of these instruments. Edge 3D imaging, LLC have developed products with real-time 3D enhanced depth of field viewing for a fraction of the cost of confocal. Our add-on systems convert your existing 2D microscopes into very powerful high resolution 3D microscopes, allowing you to observe your specimens in a whole new light. Improved Image Quality EdgeŽ proprietary optical systems enhance image quality through improved depth perception, resolution and contrast control. Our products' simplicity allows real-time inter-action, which leads to faster and better understanding of the image. With 2D microcopy critical information about 3-dimentional structures can be lost or misinterpreted. True Z-Axis Resolution The importance of Z-axis information has popularized methods such as deconvolution and confocal microscopy. Instantly understand Z-axis information without the use of computers or the need to create serial stacks. By seeing Z-axis information in real-time and in the context of a specimen's entire thickness, the viewer gains additional information about interrelationships between structures. Publications -About Edge 3D Technology Greenberg, G. (1998) A retrofit for conventional light microscopes that provides high-definition 3-D imaging in real-time. Oral presentation. Scanning Meeting 98. Baltimore, MD. Greenberg, G. and Boyde, A. (1997) Direct-view 3-D microscopy using conventional lenses. Microscopy and Analysis 22:7-9. Greenberg, G., Boyde, A. (1997) Convenient and controllable direct-view 3D imaging in conventional light microscopes: Approaches via illumination and inspection. Proc RMS 32/2:87-101 Greenberg, G., Boyde, A. Multiple oblique illumination method of high definition stereo microscopy. In Focus on Modern Microscopy. Cheng, PC (ed), World Scientific Publishing Co. PTE. LTD. (in press) Greenberg, G. (1994)Direct 3-D imaging using a multiple oblique microscope. Scanning 16:248-249 Greenberg, G. and Boyde, A. (1995) Reply to the rebuttal, Galbraith, W. (1995) Letters to the Editors Neuroimage 2, 85-87 Greenberg, G. and Boyde A. (1993) Novel method for stereo imaging in light microscopy at high magnifications. Neuroimage 1:121-128 Patents
and Patents Pending Illumination
System and Method for a High Definition Light Microscope USA Improvement in Stereoscopic Microscopes USA 5,539,572 07/29/96 Illumination System for Microscopes USA 5,548,441 08/20/96 Fiber Optic Illumination System for Microscopes USA 5,570,228 10/29/96 Illumination
System and Method for a High Definition Light Microscope USA Continuous
Center Aperturing Illumiation System for Micorscopes USA 5,684,626 Stereo Microscope Condenser USA 5,706,128 01/06/98 Illumination
System and Method for a 3D High Definition Light Microscope Center Masking Add-On Illuminator USA 5,969,856 10/19/99 Improvements in
Stereoscopic Microscopes USA 6,020,993 02/01/00
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